BleachingCorrection 20. 12. 2004

J. Rietdorf EMBL Heidelberg in Multimedia

The bleach correction macro for ImageJ corrects for bleaching or intensity fluctuations by normalizing the images of a stack to the same mean intensity. This method only works well if the mean intensity is not altered substantially (e.g. if a bright structure moves out of the imaging field). A ROI can be defined to select a suitable region as reference.


   
 
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